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Medium Detail Information

Medium No.
1019
Medium
Archaeoglobus fulgidus Medium
Composition
 KP buffer*5ml
 MgCl2·6H2O0.75g
 CaCl2·2H2O0.15g
 NH4Cl0.54g
 NaCl30g
 Na2SO42.4g
 Sodium lactate2.2g
 Bacto Yeast Extract (Difco)0.5g
 Fe(NH4)2(SO4)2·7H2O2mg
 Trace element solution**2ml
 Vitamin solution***2ml
 Resazurin1mg
 Na2CO31g
 Na2S·9H2O0.36g
 Distilled water1L
 pH unadjusted 
  Mix ingredients except KP buffer, vitamin solution, Na2CO3 and Na2S·9H2O. Dispense the medium into suitable culture vessels under a stream of N2/CO2 (80/20) and seal with butyl rubber stoppers. Separately autoclave KP buffer and Na2S·9H2O (5% solution) under a N2 atmosphere. Sterilize vitamin and 10% Na2CO3 solutions by filtration. Prior to inoculation, aseptically and anaerobically add KP buffer, vitamin, Na2CO3 and Na2S·9H2O solutions.
       
  *KP buffer
   KH2PO4119g
   K2HPO421g
   Distilled water1L
  Autoclaved under N2.
       
  **Trace element solution
   Nitrilotriacetic acid (NTA)12.8g
   FeCl3·6H2O1.35g
   MnCl2·4H2O0.1g
   CoCl2·6H2O0.024g
   CaCl2·2H2O0.1g
   ZnCl20.1g
   CuCl2·2H2O0.025g
   H3BO30.01g
   Na2MoO4·2H2O0.024g
   NaCl1g
   NiCl2·6H2O0.12g
   Na2SeO40.004g
   Na2WO40.004g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
       
  ***Vitamin solution
   Biotin2mg
   Folic acid2mg
   Pyridoxine-HCl10mg
   Thiamine-HCl5mg
   Riboflavin5mg
   Nicotinic acid5mg
   Ca-pantothenate5mg
   p-Aminobenzoic acid1mg
   Vitamin B120.01mg
   Distilled water1L
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