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Medium Detail Information

Medium No.
1021
Medium
Desulfovibrio medium
Composition
 K2HPO40.5g
 NH4Cl1g
 Na2SO41g
 CaCl2·2H2O0.1g
 MgSO4·7H2O2g
 Sodium lactate2g
 Yeast extract1g
 FeSO4·7H2O0.5g
 Resazurin1mg
 Thioglycolate solution*10ml
 Distilled water990ml
 pH 7.8 
  Dissolve ingredients except thioglycolate solution, dispense under a N2 atmosphere in culture vessels, and autoclave. Adjust the final pH of the medium to 7.8.
       
  *Thioglycolate solution
   Sodium thioglycolate0.1g
   Ascorbic acid0.1g
   Distilled water10ml
  Sterilize by filtration.
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