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Medium Detail Information

Medium No.
1028
Medium
Modified WS medium
Composition
 KH2PO40.136g
 NH4Cl0.54g
 MgCl2·6H2O3.05g
 CaCl2·2H2O0.147g
 NaCl20g
 Methanol0.8ml
 NaHCO32.52g
 Vitamin solution*2ml
 Trace element solution**1ml
 Resazurin1mg
 Cysteine-HCl0.3g
 Na2S·9H2O0.3g
 Distilled water1L
  Mix ingredients except for methanol, vitamin solution, cysteine-HCl and Na2S·9H2O, and autoclave under a N2/CO2 atmosphere (80/20). Separately autoclave cysteine-HCl and Na2S·9H2O solutions as 3% solutions under a N2 atmosphere. Sterilize methanol and vitamin solution by filtration. Prior to inoculation, add methanol, vitamin solution, cysteine-HCl and Na2S·9H2O.
       
  *Vitamin solution
   Biotin2mg
   Folic acid2mg
   Pyridoxine-HCl10mg
   Thiamine-HCl5mg
   Riboflavin5mg
   Nicotinic acid5mg
   Ca-pantothenate5mg
   p-Aminobenzoic acid1mg
   Vitamin B120.01mg
   Distilled water1L
       
  **Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   FeCl3·6H2O1.35g
   MnCl2·4H2O0.1g
   CoCl2·6H2O0.024g
   CaCl2·2H2O0.1g
   ZnCl20.1g
   CuCl2·2H2O0.025g
   H3BO30.01g
   Na2MoO4·2H2O0.024g
   NaCl1g
   NiCl2·6H2O0.12g
   Na2SeO40.004g
   Na2WO40.004g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
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