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Medium Detail Information

Medium No.	1031
Medium
Composition
Sodium pyruvate 2 g   KH2PO4 1 g   (NH4)2SO4 1 g   Na2S2O3·5H2O 0.5 g   Yeast extract 0.5 g   MgCl2·6H2O 0.2 g   CaCl2 45 mg   NaCl 0.2 g   Vitamin B12 20 μg   Nicotinic acid 10 μg   p-Aminobenzoic acid 3 μg   Trace element solution* 1 ml   Distilled water 1 L   pH 6.8       Mix ingredients except for vitamin B12, nicotinic acid and p-aminobenzoic acid, and autoclave under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add filter-sterilized vitamins.                   *Trace element solution       2Na-EDTA 5 g       FeCl2·4H2O 1 g       H3BO3 0.3 g       CoCl2·6H2O 0.11 g       ZnCl2 50 mg       MnCl2·4H2O 30 mg       Na2MoO4·2H2O 30 mg       NiSO4·6H2O 30 mg       CuCl2·2H2O 10 mg       Distilled water 1 L
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