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Medium Detail Information

Medium No.
1031
Medium
Composition
 Sodium pyruvate2g
 KH2PO41g
 (NH4)2SO41g
 Na2S2O3·5H2O0.5g
 Yeast extract0.5g
 MgCl2·6H2O0.2g
 CaCl245mg
 NaCl0.2g
 Vitamin B1220μg
 Nicotinic acid10μg
 p-Aminobenzoic acid3μg
 Trace element solution*1ml
 Distilled water1L
 pH 6.8 
  Mix ingredients except for vitamin B12, nicotinic acid and p-aminobenzoic acid, and autoclave under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add filter-sterilized vitamins.
       
  *Trace element solution
   2Na-EDTA5g
   FeCl2·4H2O1g
   H3BO30.3g
   CoCl2·6H2O0.11g
   ZnCl250mg
   MnCl2·4H2O30mg
   Na2MoO4·2H2O30mg
   NiSO4·6H2O30mg
   CuCl2·2H2O10mg
   Distilled water1L
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