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Medium Detail Information

Medium No.
1049
Medium
AS medium
Composition
  NaCl 20 g
  Bacto Yeast Extract (Difco) 2 g
  MgCl2·6H2O 3 g
  MgSO4·7H2O 6 g
  (NH4)2SO4 1 g
  CaCl2·2H2O 0.3 g
  KH2PO4 0.2 g
  KCl 0.5 g
  NaBr 0.05 g
  H3BO3 0.025 g
  SrCl2·6H2O 0.02 g
  Fe(III) ammonium citrate 0.01 g
  Bis Tris Propane 2.25 g
  Trace elements solution* 10 ml
  Vitamin solution** 10 ml
  Resazurin 1 mg
  Sulfur (powder) 10 g
  Na2S·9H2O 0.3 g
  Distilled water 1 L
 
    Mix ingredients except for vitamin, sulfur and Na2S·9H2O, adjust pH to 7 with H2SO4, and autoclave under a N2 atmosphere. Sulfur is separately sterilized by autoclaving for 60 min at 105°C on each of 3 successive days. Separately autoclave Na2S·9H2O as 5% solution under a N2 atmosphere. Anaerobically add filter-sterile vitamin, sulfur and Na2S·9H2O.
             
    *Trace elements solution
      Nitrilotriacetic acid (NTA) 4.1 g
      FeCl3·6H2O 0.36 g
      MnCl2·4H2O 0.09 g
      CoCl2·6H2O 0.15 g
      CaCl2·2H2O 0.018 g
      ZnCl2 0.09 g
      H3BO3 0.017 g
      Na2MoO4·2H2O 0.009 g
      NiCl2·6H2O 0.018 g
      Na2SeO3 1.6 mg
      Na2WO4 1.6 mg
      AlK(SO4)2 5 mg
      Distilled water 1 L
 
    First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add other minerals. Final pH 7.0.
             
    **Vitamin solution
      Biotin 2 mg
      Folic acid 2 mg
      Pyridoxine-HCl 10 mg
      Thiamine-HCl 5 mg
      Riboflavin 5 mg
      Nicotinic acid 5 mg
      Ca-pantothenate 5 mg
      p-Aminobenzoic acid 1 mg
      Vitamin B12 0.01 mg
      Distilled water 1 L
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