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Medium Detail Information

Medium No.
1055
Medium
Composition
 Glucose5g
 Polypepton5g
 Trypticase Peptone (BBL)5g
 Yeast extract10g
 CaCl2·2H2O0.01g
 MgSO4·7H2O0.02g
 KH2PO40.04g
 NaCl0.08g
 K2HPO40.04g
 NaHCO30.4g
 Cysteine-HCl1g
 Resazurin1mg
 Distilled water1L
 pH 7 
  Mix ingredients except for cysteine-HCl. Dispense the medium into suitable culture vessels under a N2/CO2 (80/20) atmosphere, and seal with butyl rubber stoppers. Sterilze cysteine-HCl solution by filtration. Prior to inoculation, aseptically and anaerobically add cysteine-HCl solution.
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