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Medium Detail Information

Medium No.
1084
Medium
Actinotalea fermentas medium
Composition
 K2HPO42.21g
 KH2PO41.5g
 (NH4)2SO41.3g
 MgCl2·6H2O0.1g
 CaCl2·2H2O0.02g
 FeSO4·7H2O1.25mg
 Yeast extract5g
 Cellobiose or Cellulose (MN 300)5g
 NaHCO30.8g
 Resazurin1mg
 Cysteine-HCl0.5g
 Distilled water1L
 Adjust pH to 7.4 with 8 M NaOH 
  Prepare the medium anaerobically under 100% nitrogen. Cellobiose, NaHCO3, and cysteine-HCl are sterilized separately by autoclaving under N2. For aerobic growth, omit resazurin and cysteine-HCl.
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