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Medium No.	1096
Medium	1/5 Marine Agar with Phenanthrene
Composition
Bacto Marine Broth 2216 (Difco) 7.5 g   Sodium pyruvate 5 g   Agar (if needed) 15 g   Sea water* 800 ml   Distilled water 200 ml   pH 7.6       Layered the Phenanthrene-Agar*** on the 1/5 Marine Agar plate.                   *Filtered, aged seawater or artificial seawater (Daigo's Artificial Seawater SP**)                   **Wako Pure Chemical Ind., Ltd., Osaka, Japan.                   ***Phenanthrene-Agar       Phenanthrene 51 mg       Ethanol 6 ml       1/5 Marine Agar 100 ml     Sterilize separately by filtration.
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