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Medium Detail Information

Medium No.
1110
Medium
Alkalic MRS (pH 8.5) + 3% NaCl
Composition
 Solution A*900ml
 Solution B**100ml
  Separately autoclave Solutions A and B. Mix aseptically. Final pH around 8.5.
       
  *Solution A
   Peptone10g
   Meat extract10g
   Yeast extract5g
   Glucose20g
   Tween 801g
   K2HPO42g
   Sodium acetate5g
   Diammonium hydrogen citrate2g
   MgSO4·7H2O0.2g
   MnSO4·nH2O0.05g
   NaCl30g
   Agar (if needed)15g
   Distilled water900ml
 pH unadjusted 
       
  **Solution B
   Na2CO36g
   Distilled water100ml
 pH unadjusted 
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