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Medium No.	1113
Medium	Sporulation Medium
Composition
Dissolve each regent in distilled water and sterilize them individually. Cool them down to about 60℃, and mix all the solution together.     Solution A       Nutrient broth 8 g       FeSO4·7H2O 0.278 mg       MgSO4·7H2O 0.25 g       KCl 1 g       Agar (if needed) 15 g       Distilled water 800 ml   pH unadjusted       Solution B       Ca(NO3)2·4H2O 236.15 mg       Distilled water 100 ml   pH unadjusted       Solution C       MnCl2·4H2O 1.979 mg       Distilled water 100 ml   pH unadjusted
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