Search Results Detail

Medium Detail Information

Medium No.
1124
Medium
Thermus medium
Composition
 Bacto Yeast Extract (Difco)1g
 Bacto Tryptone (Difco)1g
 Sodium glutamate monohydrate1g
 CaSO4·2H2O0.06g
 MgSO4·7H2O0.1g
 NaCl0.008g
 KNO30.1g
 NaNO30.69g
 Na2HPO40.11g
 Na2S2O3·5H2O1.24g
 Trace elements solution*10ml
 Gelrite (if needed)8g
 Distilled water1L
 Adjust pH to 7.5 with NaOH 
  For solid medium, equal volumes of separately autoclaved 1.6% gelrite and double-strength liquid medium containing 2 g/L MgSO4·7H2O are mixed.
       
  *Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   FeCl3·6H2O1.35g
   MnCl2·4H2O0.1g
   CoCl2·6H2O0.024g
   CaCl2·2H2O0.1g
   ZnCl20.1g
   CuCl2·2H2O0.025g
   H3BO30.01g
   Na2MoO4·2H2O0.024g
   NaCl1g
   NiCl2·6H2O0.12g
   Na2SeO40.004g
   Na2WO40.004g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
Comment