| | KH2PO4 | 0.5 | g |
| | MgSO4·7H2O | 0.2 | g |
| | NaCl | 0.4 | g |
| | NH4Cl | 0.6 | g |
| | CaCl2·2H2O | 0.05 | g |
| | Sodium pyruvate | 1 | g |
| | Sodium succinate | 1 | g |
| | Sodium acetate | 1 | g |
| | Peptone | 0.3 | g |
| | Yeast extract | 0.3 | g |
| | Ferric citrate solution* (0.1%) | 5 | ml |
| | Trace element solution SL7** | 1 | ml |
| | NaHCO3 (3% (w/v)) | 50 | ml |
| | Vitamin B12 (0.002% (w/v)) | 1 | ml |
| | Na2S2O3·5H2O | 0.248 | g |
| | Na2S·9H2O | 0.24 | g |
| | Resazurin | 1 | mg |
| | Distilled water | 1 | L |
| | pH 7 | |
|
| | | Mix ingredients except for vitamin B12, NaHCO3 and Na2S·9H2O, and autoclave under a N2 atmosphere. Separately autoclave Na2S·9H2O solution under a N2 atmosphere. Sterilize vitamin B12 and NaHCO3 solutions by filtration. Prior to inoculation, aseptically and anaerobically add them. For strain JA492, inoculum size should be higher than 10 %. |
| | | | | | | |
| | | *Soluble in hot water. |
| | | | | | | |
| | | **Trace element solution SL7 |
| | | | HCl (25%; 7.7 M) | 10 | ml |
| | | | FeCl2·4H2O | 1.5 | g |
| | | | ZnCl2 | 70 | mg |
| | | | MnCl2·4H2O | 100 | mg |
| | | | H3BO3 | 60 | mg |
| | | | CoCl2·6H2O | 200 | mg |
| | | | CuCl2·2H2O | 20 | mg |
| | | | NiCl2·6H2O | 20 | mg |
| | | | Na2MoO4·2H2O | 40 | mg |
| | | | Distilled water | 990 | ml |
|
| | | Initially, dissolve FeCl2·4H2O in 25% HCl, then add distilled water followed by the other components. |