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Medium Detail Information

Medium No.
1168
Medium
Methanococcoides medium
Composition
 KCl0.335g
 MgCl2·6H2O4g
 MgSO4·7H2O3.45g
 NH4Cl0.24g
 CaCl2·2H2O0.14g
 K2HPO40.14g
 NaCl18g
 Vitamin solution*10ml
 Trace elements solution**10ml
 Fe(NH4)2(SO4)2·7H2O2mg
 Sodium acetate1g
 Bacto Yeast Extract (Difco)2g
 Polypeptone2g
 Trimethylamine-HCl3g
 Resazurin1mg
 NaHCO35g
 Cysteine-HCl0.5g
 Na2S·9H2O0.5g
 Distilled water1L
  Mix ingredients except for vitamin solution, cysteine-HCl and Na2S·9H2O, and autoclave under a N2/CO2 atmosphere (80/20). Separately autoclave cysteine-HCl and Na2S·9H2O solutions as 5% solutions under a N2 atmosphere. Prior to inoculation, add the filter-sterile vitamin solution, cysteine-HCl and Na2S·9H2O.
       
  *Vitamin solution
   Biotin2mg
   Folic acid2mg
   Pyridoxine-HCl10mg
   Thiamine-HCl5mg
   Riboflavin5mg
   Nicotinic acid5mg
   Ca-pantothenate5mg
   p-Aminobenzoic acid1mg
   Vitamin B120.01mg
   Distilled water1L
       
  **Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   FeCl3·6H2O1.35g
   MnCl2·4H2O0.1g
   CoCl2·6H2O0.024g
   CaCl2·2H2O0.1g
   ZnCl20.1g
   CuCl2·2H2O0.025g
   H3BO30.01g
   Na2MoO4·2H2O0.024g
   NaCl1g
   NiCl2·6H2O0.12g
   Na2SeO40.004g
   Na2WO40.004g
   KAl(SO4)2·12H2O0.02g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
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