Search Results Detail

Medium Detail Information

Medium No.
1170
Medium
Methanohalophilus medium
Composition
 NH4Cl0.33g
 KH2PO40.33g
 KCl0.33g
 CaCl2·2H2O0.33g
 MgCl2·6H2O0.33g
 K2SO40.01g
 NaCl70g
 Vitamin solution*10ml
 Trace elements solution**10ml
 Bacto Yeast Extract (Difco)0.05g
 Methylamine-HCl5g
 Resazurin1mg
 NaHCO35g
 Cysteine-HCl0.3g
 Na2S·9H2O0.15g
 Distilled water1L
  Mix ingredients except for vitamin solution, NaHCO3, cysteine-HCl and Na2S·9H2O, and autoclave under a N2/CO2 atmosphere (80/20). Separately autoclave cysteine-HCl and Na2S·9H2O solutions as 3% and 1.5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, add the filter-sterile vitamin, filter-sterile 5% NaHCO3 solution, cysteine-HCl and Na2S·9H2O solutions. Final pH of the medium is approximately 7.2.
       
  *Vitamin solution
   Biotin2mg
   Folic acid2mg
   Pyridoxine-HCl10mg
   Thiamine-HCl5mg
   Riboflavin5mg
   Nicotinic acid5mg
   Ca-pantothenate5mg
   p-Aminobenzoic acid1mg
   Vitamin B120.01mg
   Distilled water1L
       
  **Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   FeCl3·6H2O1.35g
   MnCl2·4H2O0.1g
   CoCl2·6H2O0.024g
   CaCl2·2H2O0.1g
   ZnCl20.1g
   CuCl2·2H2O0.025g
   H3BO30.01g
   Na2MoO4·2H2O0.024g
   NaCl1g
   NiCl2·6H2O0.12g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
Comment