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Medium Detail Information

Medium No.	1178
Medium
Composition
NH4NO3 0.1 g   KH2PO4 0.01 g   Fe(III)-EDTA 2.5 mg   Vitamin solution* 10 ml   Trace elements solution** 10 ml   Seawater*** 980 ml   pH 8.0       Mix ingredients, adjust pH to 8 with NaOH, and sterilize by filtration. Add filter-sterilized methane gas to the vessel. Methane and air ratio in the headspace is about 1:4. The culture was incubated at 20°C with shaking at 100 r.p.m.                   *Vitamin solution       Vitamin B12 0.275 mg       Biotin 0.25 mg       Thiamine-HCl 50 mg       Distilled water 1 L                   **Trace elements solution       2Na-EDTA 37.2 mg       CuSO4·5H2O 0.025 mg       ZnSO4·7H2O 0.575 mg       MnCl2·4H2O 0.455 mg       CoCl2·6H2O 0.06 mg       (NH4)6Mo7O24·4H2O 0.027 mg       Distilled water 1 L                   ***Natural seawater or artificial seawater (e.g. Daigo's Artificial Seawater SP, Wako Pure Chemical Ind., Ltd., Osaka, Japan).
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