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Medium Detail Information

Medium No.
1178
Medium
Composition
 NH4NO30.1g
 KH2PO40.01g
 Fe(III)-EDTA2.5mg
 Vitamin solution*10ml
 Trace elements solution**10ml
 Seawater***980ml
 pH 8.0 
  Mix ingredients, adjust pH to 8 with NaOH, and sterilize by filtration. Add filter-sterilized methane gas to the vessel. Methane and air ratio in the headspace is about 1:4. The culture was incubated at 20°C with shaking at 100 r.p.m.
       
  *Vitamin solution
   Vitamin B120.275mg
   Biotin0.25mg
   Thiamine-HCl50mg
   Distilled water1L
       
  **Trace elements solution
   2Na-EDTA37.2mg
   CuSO4·5H2O0.025mg
   ZnSO4·7H2O0.575mg
   MnCl2·4H2O0.455mg
   CoCl2·6H2O0.06mg
   (NH4)6Mo7O24·4H2O0.027mg
   Distilled water1L
       
  ***Natural seawater or artificial seawater (e.g. Daigo's Artificial Seawater SP, Wako Pure Chemical Ind., Ltd., Osaka, Japan).
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