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Medium Detail Information

Medium No.	1192
Medium	Thermotoga lettingae medium
Composition
K2HPO4 0.3 g   KH2PO4 0.3 g   NH4Cl 1 g   MgCl2·6H2O 0.2 g   CaCl2·2H2O 0.1 g   KCl 0.1 g   NaCl 10 g   Na2S2O3·5H2O 5 g   Bacto Yeast Extract (Difco) 0.5 g   Glucose 4 g   Na2CO3 2 g   Trace element solution* 10 ml   Resazurin 1 mg   Cysteine-HCl 0.5 g   Na2S·9H2O 0.5 g   Distilled water 1 L     Mix ingredients except for glucose, Na2CO3, cysteine-HCl and Na2S·9H2O, and autoclave under a N2/CO2 atmosphere (80/20). Separately autoclave cysteine-HCl (as 5% solution) and Na2S·9H2O solutions (as 5% solution) under a N2 atmosphere. Prior to inoculation, add filter-sterile glucose and Na2CO3 solutions, cysteine-HCl, and Na2S·9H2O. Final pH of the complete medium should be 7.0.                   *Trace elements solution       Nitrilotriacetic acid (NTA) 12.8 g       FeCl3·6H2O 1.35 g       MnCl2·4H2O 0.1 g       CoCl2·6H2O 0.024 g       CaCl2·2H2O 0.1 g       ZnCl2 0.1 g       CuCl2·2H2O 0.025 g       H3BO3 0.01 g       Na2MoO4·2H2O 0.024 g       NaCl 1 g       NiCl2·6H2O 0.12 g       KAl(SO4)2·12H2O 0.018 g       Na2SeO4 0.004 g       Na2WO4 0.004 g       Distilled water 1 L     First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
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