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Medium Detail Information

Medium No.
1199
Medium
Thermotoga thermarum Medium
Composition
 Soluble starch5g
 Bacto Yeast Extract (Difco)0.5g
 KH2PO40.5g
 NaCl3.5g
 MgSO4·7H2O0.88g
 MgCl2·6H2O0.69g
 CaCl2·2H2O0.14g
 KCl0.08g
 Na2-EDTA0.768g
 Trace minerals*10ml
 (NH4)2Ni(SO4)23mg
 NaBr12.5mg
 H3BO33.8mg
 SrCl2·6H2O1.9mg
 KI0.006mg
 Resazurin1mg
 Na2S·9H2O**0.5g
 Distilled water1L
  Mix ingredients except Na2S·9H2O, adjust pH to 7.0 with NaOH and autoclaved under a N2 atmosphere. Separately autoclave and neutralize Na2S·9H2O as 5% solution under a N2 atmosphere. Anaerobically add Na2S·9H2O solution.
       
  *Trace minerals
   Nitrilotriacetic acid1.5g
   MgSO4·7H2O3g
   MnSO4·2H2O0.5g
   NaCl1g
   FeSO4·7H2O0.1g
   CoSO4·7H2O0.18g
   CaCl2·2H2O0.1g
   ZnSO4·7H2O0.18g
   CuSO4·5H2O0.01g
   H3BO30.01g
   Na2MoO4·2H2O0.01g
   NiCl2·6H2O0.025g
   Na2SeO3·5H2O0.8mg
   Na2WO40.8mg
   KAl(SO4)2·12H2O4mg
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).
       
  **The sulfide is prepared in a vessel sealed with a butyl rubber stopper. Autoclave under a N2 atmosphere. After cooling, the pH is adjusted to about 7.3 by adding of sterile 4-0.2 N H2SO4 drop-wise with a syringe without opening the bottle. Appearance of yellow color indicate the pH is about 8. The vessel should be shaken continuously to avoid precipitation of elemental sulfur. The final solution should be transparent yellow.
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