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Medium Detail Information

Medium No.
1203
Medium
LB + Ampicillin, Hygromycin medium
Composition
  Bacto Tryptone (Difco) 10 g
  Yeast extract 5 g
  NaCl 5 g
  Ampicillin solution (50 mg/ml)* 1 ml
  Hygromycin B solution (50 mg/ml)* 3 ml
  Distilled water 1 L
  Agar (if needed) 15 g
  pH 7.0  
             
    *Sterilize separately by filtration.
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