|
NaCl |
16 |
g |
|
MgSO4·7H2O |
3.9 |
g |
|
MgCl2·6H2O |
3.9 |
g |
|
CaCl2·2H2O |
0.14 |
g |
|
KCl |
0.65 |
g |
|
NaHCO3 |
0.5 |
g |
|
NH4Cl |
0.1 |
g |
|
(NH4)2SO4 |
1 |
g |
|
MES |
1 |
g |
|
KH2PO4 |
0.1 |
g |
|
NaBr |
0.1 |
g |
|
Yeast extract |
1 |
g |
|
Sodium pyruvate |
1 |
g |
|
Na2SiO3 |
10 |
mg |
|
H3BO3 |
30 |
mg |
|
SrCl2·6H2O |
15 |
mg |
|
KI |
0.05 |
mg |
|
NaNO3 |
0.05 |
mg |
|
Trace element solution* |
10 |
ml |
|
Vitamin solution** |
1 |
ml |
|
Distilled water |
1000 |
ml |
|
pH 7.5 |
|
|
|
|
Mix ingredients except for sodium pyruvate, vitamin and bicarbonate. Dispense the medium into suitable culture vessels under a N2 atmosphere, seal with butyl rubber stoppers, and autoclave at 121°C for 15 min. Aseptically and anaerobically add filter-sterilized sodium pyruvate, vitamin and bicarbonate solutions, and adjust pH to 7.5 using compact pH meter. |
|
|
|
|
|
|
|
|
|
*Trace element solution |
|
|
|
EDTA |
0.5 |
g |
|
|
|
FeSO4·7H2O |
0.2 |
g |
|
|
|
ZnSO4·7H2O |
10 |
mg |
|
|
|
MnCl2·4H2O |
3 |
mg |
|
|
|
H3BO3 |
30 |
mg |
|
|
|
CoCl2·6H2O |
20 |
mg |
|
|
|
CuCl2·2H2O |
1 |
mg |
|
|
|
NiCl2·6H2O |
2 |
mg |
|
|
|
Na2MoO4·2H2O |
3 |
mg |
|
|
|
Distilled water |
1 |
L |
|
|
|
|
|
|
|
|
|
**Vitamin solution |
|
|
|
Biotin |
2 |
mg |
|
|
|
Folic acid |
2 |
mg |
|
|
|
Pyridoxine-HCl |
10 |
mg |
|
|
|
Thiamine-HCl |
5 |
mg |
|
|
|
Riboflavin |
5 |
mg |
|
|
|
Nicotinic acid |
5 |
mg |
|
|
|
Ca-pantothenate |
5 |
mg |
|
|
|
Vitamin B12 |
0.1 |
mg |
|
|
|
p-Aminobenzoic acid |
5 |
mg |
|
|
|
Lipoic acid |
5 |
mg |
|
|
|
Distilled water |
1 |
L |
|