カタログ検索結果 詳細情報

Medium Detail Information

Medium No.	1251
Medium	Medium for Strain KKC1
Composition
NaCl 16 g   MgSO4·7H2O 3.9 g   MgCl2·6H2O 3.9 g   CaCl2·2H2O 0.14 g   KCl 0.65 g   NaHCO3 0.5 g   NH4Cl 0.1 g   (NH4)2SO4 1 g   MES 1 g   KH2PO4 0.1 g   NaBr 0.1 g   Yeast extract 1 g   Sodium pyruvate 1 g   Na2SiO3 10 mg   H3BO3 30 mg   SrCl2·6H2O 15 mg   KI 0.05 mg   NaNO3 0.05 mg   Trace element solution* 10 ml   Vitamin solution** 1 ml   Distilled water 1000 ml   pH 7.5         Mix ingredients except for sodium pyruvate, vitamin and bicarbonate. Dispense the medium into suitable culture vessels under a N2 atmosphere, seal with butyl rubber stoppers, and autoclave at 121°C for 15 min. Aseptically and anaerobically add filter-sterilized sodium pyruvate, vitamin and bicarbonate solutions, and adjust pH to 7.5 using compact pH meter.                   *Trace element solution       EDTA 0.5 g       FeSO4·7H2O 0.2 g       ZnSO4·7H2O 10 mg       MnCl2·4H2O 3 mg       H3BO3 30 mg       CoCl2·6H2O 20 mg       CuCl2·2H2O 1 mg       NiCl2·6H2O 2 mg       Na2MoO4·2H2O 3 mg       Distilled water 1 L                   **Vitamin solution       Biotin 2 mg       Folic acid 2 mg       Pyridoxine-HCl 10 mg       Thiamine-HCl 5 mg       Riboflavin 5 mg       Nicotinic acid 5 mg       Ca-pantothenate 5 mg       Vitamin B12 0.1 mg       p-Aminobenzoic acid 5 mg       Lipoic acid 5 mg       Distilled water 1 L
Comment