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Medium Detail Information

Medium No.	1264
Medium
Composition
Glucose 5 g   Yeast extract 7.5 g   NaCl 3 g   NH4Cl 2.5 g   K2HPO4 2.5 g   Sodium pyruvate 0.1 g   TAPS* 24 g   Na2CO3 solution** (1.0 M) 60 ml   Distilled water 940 ml   Gellan gum (if needed) 10 g     Mix ingredients except Na2CO3 solution, and autoclave at 121℃ for 20 min.                   *N-Tris hydroxymethyl methyl-3-aminopropanesulfonic acid)                   **After autoclaving, aseptically add 1 M Na2CO3 solution to the medium. Check final pH to be about 8.5.
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