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Medium Detail Information

Medium No.
1264
Medium
Composition
 Glucose5g
 Yeast extract7.5g
 NaCl3g
 NH4Cl2.5g
 K2HPO42.5g
 Sodium pyruvate0.1g
 TAPS*24g
 Na2CO3 solution** (1.0 M)60ml
 Distilled water940ml
 Gellan gum (if needed)10g
  Mix ingredients except Na2CO3 solution, and autoclave at 121℃ for 20 min.
       
  *N-Tris hydroxymethyl methyl-3-aminopropanesulfonic acid)
       
  **After autoclaving, aseptically add 1 M Na2CO3 solution to the medium. Check final pH to be about 8.5.
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