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Medium Detail Information

Medium No.	1270
Medium	NMS modified for Gela4
Composition
NH4Cl 1 g   MgSO4·6H2O 1 g   CaCl2·2H2O 0.2 g   Fe·EDTA 0.038 g   Phosphate solution* 10 ml   Trace element solution** 0.5 ml   Methanol 5 ml   Agar (if needed) 15 g   Seawater*** 1 L                   *Phosphate solution       Na2HPO4·12H2O 71.6 g       KH2PO4 26 g       Distilled water 1 L   Adjust pH to 6.8                     **Trace element solution       CuSO4·5H2O 1 g       FeSO4·7H2O 2.5 g       ZnSO4·7H2O 2 g       H3BO3 0.075 g       CoCl2·6H2O 0.25 g       EDTA·2Na 1.25 g       MnCl2·4H2O 0.1 g       NiCl2·6H2O 0.05 g       Na2MoO4·2H2O 1.3 g       Distilled water 5 L                   ***Filtered, aged seawater or artificial seawater (Daigo's Artificial Seawater SP****).                   **** Wako Pure Chemical Ind., Ltd., Osaka, Japan.
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