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Medium Detail Information

Medium No.
 1278
Medium
Composition
 Yeast extract*1g
 Na2S2O3·5H2O5g
 KH2PO41.5g
 Na2HPO44.5g
 MgSO4·7H2O0.1g
 NH4Cl0.3g
 Sodium aspartate1g
 Trace metal solution**5ml
 Distilled water1L
 Agar (if needed)15g
 pH 6.0 
       
  *Yeast extract
  Sterilize by filtration and add to the medium sterilized by autoclaving.
       
  **Trace metal solution
   2Na-EDTA50g
   ZnSO4·7H2O22g
   CaCl2·2H2O5.54g
   MnCl2·4H2O5.06g
   FeSO4·7H2O4.99g
   (NH4)6Mo7O24·4H2O1.1g
   CuSO4·5H2O1.57g
   CoCl2·6H2O1.61g
   Distilled water1L
 pH 6.0 
  Adjusted to pH 6.0 with KOH.
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