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Medium Detail Information

Medium No.	1289
Medium	SL medium
Composition
KH2PO4 0.35 g   K2HPO4 0.35 g   NH4Cl 1 g   MgCl2·6H2O 0.5 g   CaCl2·2H2O 0.1 g   KCl 0.2 g   NaCl 26 g   PIPES 3.4 g   Sodium acetate 1.6 g   Bacto Yeast Extract (Difco) 2 g   Bacto Peptone (Difco) 2 g   Maltose 3.5 g   Resazurin 0.5 mg   Na2S·9H2O 0.5 g   Distilled water 1 L     Mix ingredients except for maltose and sodium sulfide, adjust pH to 6.0 with NaOH and autoclave under a N2 atmosphere. Concentrated sulfide solution is sterilized separately under a N2 atmosphere. Prior to inoculation, add the filter-sterile maltose and sulfide. Final pH of the medium is 6.0.
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