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Medium Detail Information

Medium No.
1324
Medium
HS TMA
Composition
 KH2PO40.68g
 NH4Cl1g
 MgCl2·6H2O11g
 CaCl2·2H2O0.3g
 NaCl23.4g
 KCl1g
 NaHCO32.5g
 Bacto Yeast Extract (Difco)2g
 Sodium acetate3.3g
 Trimethylamine-HCl0.96g
 Vitamin solution*10ml
 Trace element solution**10ml
 Resazurin1mg
 Cysteine-HCl0.5g
 Na2S·9H2O0.25g
 Distilled water1L
  Mix ingredients except for vitamin solution, trimethylamine-HCl, yeast extract, sodium acetate, cysteine-HCl and Na2S·9H2O, and autoclave under a N2/CO2 atmosphere (80/20). After autoclaving, a precipitate will dissolve by shaking. Concentrated trimethylamine-HCl, yeast extract, sodium acetate, cysteine-HCl and sodium sulfide solutions are sterilized separately under a N2 atmosphere in tightly closed vessels. Prior to inoculation, add the filter-sterile vitamin solution, trimethylamine-HCl, yeast extract, sodium acetate, cysteine-HCl and Na2S·9H2O.
       
  *Vitamin solution
   Biotin2mg
   Folic acid2mg
   Pyridoxine-HCl10mg
   Thiamine-HCl5mg
   Riboflavin5mg
   Nicotinic acid5mg
   Ca-pantothenate5mg
   p-Aminobenzoic acid1mg
   Vitamin B120.01mg
   Distilled water1L
       
  **Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   FeCl3·6H2O1.35g
   MnCl2·4H2O0.1g
   CoCl2·6H2O0.024g
   CaCl2·2H2O0.1g
   ZnCl20.1g
   CuCl2·2H2O0.025g
   H3BO30.01g
   Na2MoO4·2H2O0.024g
   NaCl1g
   NiCl2·6H2O0.12g
   Na2SeO40.004g
   Na2WO40.004g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
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