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Medium No.	1339
Medium	M9-Phenanthrene
Composition
Agar solution* 900 ml   10xM9 solution** 100 ml   MgSO4 solution (1 M)*** 2.1 ml   CaCl2 solution (0.1 M)*** 1 ml   Phenanthrene solution (250 mM) in dimethyl sulfoxide (DMSO)**** 10 ml     After autoclaving of agar solution, aseptically mix 10xM9, MgSO4 and CaCl2 solutions. After cooling to 65°C, aseptically add phenanthrene solution, mix by sonication for approximately 30 seconds and pour into the plates.                   *Agar solution     Dissolve 15 g of agar in 900 ml of distilled water and sterilize by autoclaving.                   **10xM9 solution       KH2PO4 30 g       Na2HPO4 60 g       NH4Cl 10 g       NaCl 5 g       Distilled water 1 L     Sterilize separately by autoclaving.                   ***Dissolve in distilled water and sterilize by autoclaving.                   ****Sterilized by filtration. The use of DMSO-safe filters is recommended.
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