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Medium Detail Information

Medium No.
1339 
Medium
M9-Phenanthrene 
Composition
 Agar solution*900ml
 10xM9 solution**100ml
 MgSO4 solution (1 M)***2.1ml
 CaCl2 solution (0.1 M)***1ml
 Phenanthrene solution (250 mM) in dimethyl sulfoxide (DMSO)****10ml
  After autoclaving of agar solution, aseptically mix 10xM9, MgSO4 and CaCl2 solutions. After cooling to 65°C, aseptically add phenanthrene solution, mix by sonication for approximately 30 seconds and pour into the plates.
       
  *Agar solution
  Dissolve 15 g of agar in 900 ml of distilled water and sterilize by autoclaving.
       
  **10xM9 solution
   KH2PO430g
   Na2HPO460g
   NH4Cl10g
   NaCl5g
   Distilled water1L
  Sterilize separately by autoclaving.
       
  ***Dissolve in distilled water and sterilize by autoclaving.
       
  ****Sterilized by filtration. The use of DMSO-safe filters is recommended.
 
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