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Medium Detail Information

Medium No.	1343
Medium	Medium for Strain HA5
Composition
MgCl2·6H2O 0.2 g   CaCl2·2H2O 0.1 g   NH4Cl 0.1 g   KH2PO4 0.1 g   KCl 0.1 g   Trace element mixture* 1 ml   Selenite-tungstate solution** 1 ml   Vitamin solution*** 1 ml   Bicarbonate solution**** 15-30 ml   Thiosulfate solution***** 20 ml   Distilled water 950 ml   pH 7.0-7.2         Mix ingredients except for bicarbonate, vitamin and thiosulfate solutions. Dispense the medium into suitable culture vessels, seal with butyl rubber stoppers, and autoclave at 121°C for 15 min. Aseptically add other ingredients using sterile syringes, and adjust pH to 7.0-7.2 using compact pH meter.                   *Trace element mixture       HCl (25%; 7.7 M) 12.5 ml       FeSO4·7H2O 2100 mg       H3BO3 30 mg       MnCl2·4H2O 100 mg       CoCl2·6H2O 190 mg       NiCl2·6H2O 24 mg       CuCl2·2H2O 2 mg       ZnSO4·7H2O 144 mg       Na2MoO4·2H2O 36 mg       Distilled water 987 ml       Sterilized by autoclaving.                   **Selenite-tungstate solution       NaOH 0.4 g       Na2SeO3·5H2O 6 mg       Na2WO4·2H2O 8 mg       Distilled water 1 L       Sterilized by autoclaving.                   ***Vitamine solution       Biotin 2 mg       Folic acid 2 mg       Pyridoxine-HCl 10 mg       Thiamine-HCl 5 mg       Riboflavin 5 mg       Nicotinic acid 5 mg       Ca-pantothenate 5 mg       Vitamin B12 0.1 mg       p-Aminobenzoic acid 5 mg       Lipoic acid 5 mg       Distilled water 1 L       Sterilized by filtration.                   ****Bicarbonate solution (1.0 M)       NaHCO3 8.4 g       Distilled water 100 ml       Saturated with CO2 gas. Tightly sealed in bottles filled with CO2 gas and then sterilized by autoclaving.                   *****Thiosulfate solution (1.0 M)       Na2S2O3·5H2O 24.8 g       Distilled water 100 ml       Sterilized by filtration and stored under N2 gas.
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