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Medium Detail Information

Medium No.
1353
Medium
M9 medium
Composition
 10xM9 solution*100ml
 MgSO4 solution (1 M)**2.1ml
 CaCl2 solution (0.1 M)**1ml
 Distilled water900ml
  After autoclaving of distilled water, aseptically mix 10xM9, MgSO4 and CaCl2 solutions.
       
  *10xM9 solution
   KH2PO430g
   Na2HPO460g
   NH4Cl10g
   NaCl5g
   Distilled water1L
  Sterilize separately by autoclaving.
       
  **Dissolve in distilled water and sterilize by autoclaving.
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