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Medium Detail Information

Medium No.	1374
Medium	Sulfur-oxidizing bacterium medium
Composition
MgCl2·6H2O 0.2 g   CaCl2·2H2O 0.1 g   NH4Cl 0.1 g   KH2PO4 0.1 g   KCl 0.1 g   MOPS 4.2 g   Na2S2O3·5H2O 5 g   Trace elements solution* 2 ml   Bicarbonate solution (1.0 M) 30 ml   Distilled water 1 L   pH 7.0       Mix ingredients except for bicarbonate, and adjust pH to 7.0 with NaOH. Dispense the medium into suitable culture vessels, and autoclave at 121°C for 20 min. Aseptically add filter-sterile bicarbonate solution. Final pH is 7.0.                   *Trace elements solution       Nitrilotriacetic acid (NTA) 12.8 g       FeCl3·6H2O 1.35 g       MnCl2·4H2O 0.1 g       CoCl2·6H2O 0.024 g       CaCl2·2H2O 0.1 g       ZnCl2 0.1 g       CuCl2·2H2O 0.025 g       H3BO3 0.01 g       Na2MoO4·2H2O 0.024 g       NaCl 1 g       NiCl2·6H2O 0.12 g       Na2SeO4 0.004 g       Na2WO4 0.004 g       Distilled water 1 L     First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
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