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Medium Detail Information

Medium No.	1379
Medium	Medium for Acidithiobacillus ferrooxidans
Composition
(NH4)2SO4 2 g   K2HPO4 0.5 g   KCl 0.1 g   Solution I* 10 ml   Solution II** 100 ml   Distilled water 890 ml     Mix ingredients except for solutions I and II, adjust pH to 3.0 with 6 N H2SO4 and sterilize by autoclaving at 112°C for 30 min. Prior to inoculation, mix separately autoclaved solutions I and II.                   *Solution I       MgSO4·7H2O 50 g       Ca(NO3)2·4H2O 1.44 g       Distilled water 1 L     Mix ingredients, adjust pH to 3.0 with 6 N H2SO4 and sterilize by autoclaving at 112°C for 30 min.                   **Solution II       FeSO4·7H2O 400 g       0.25 N H2SO4 1 L     Sterilize by autoclaving at 112°C for 30 min.
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