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Medium Detail Information

Medium No.
1379
Medium
Medium for Acidithiobacillus ferrooxidans
Composition
 (NH4)2SO42g
 K2HPO40.5g
 KCl0.1g
 Solution I*10ml
 Solution II**100ml
 Distilled water890ml
  Mix ingredients except for solutions I and II, adjust pH to 3.0 with 6 N H2SO4 and sterilize by autoclaving at 112°C for 30 min. Prior to inoculation, mix separately autoclaved solutions I and II.
       
  *Solution I
   MgSO4·7H2O50g
   Ca(NO3)2·4H2O1.44g
   Distilled water1L
  Mix ingredients, adjust pH to 3.0 with 6 N H2SO4 and sterilize by autoclaving at 112°C for 30 min.
       
  **Solution II
   FeSO4·7H2O400g
   0.25 N H2SO41L
  Sterilize by autoclaving at 112°C for 30 min.
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