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Medium Detail Information

Medium No.	1380
Medium	Halo 2g
Composition
Glucose 1.8 g   Bacto Yeast Extract (Difco) 0.1 g   Casamino acids 0.1 g   Sodium glutamate 1 g   Trisodium citrate 1 g   KCl 2 g   K2HPO4 0.3 g   CaCl2·2H2O 0.15 g   NH4Cl 1 g   MgSO4·7H2O 50 g   NaCl 200 g   Trace elements solution* 2 ml   Agar (if needed) 20 g   Distilled water 1 L   pH 7.0-7.4       After autoclaving, add filter-sterile glucose solution.                   *Trace elements solution       Nitrilotriacetic acid (NTA) 12.8 g       FeCl3·6H2O 1.35 g       MnCl2·4H2O 0.1 g       CoCl2·6H2O 0.024 g       CaCl2·2H2O 0.1 g       ZnCl2 0.1 g       CuCl2·2H2O 0.025 g       H3BO3 0.01 g       Na2MoO4·2H2O 0.024 g       NaCl 1 g       NiCl2·6H2O 0.12 g       Distilled water 1 L     First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
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