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Medium No.	1382
Medium	VL55 GY agar
Composition
2x GY agar* 500 ml   2x VL55 buffer (pH5.5)** 500 ml     Equal volumes of separately autoclaved solutions are mixed gently.                   *2x GY agar       Glucose 2 g       Bacto Yeast Extract (Difco) 1 g       Agar 43 g       Distilled water 1 L   pH unadjusted                     **2x VL55 buffer (pH5.5)       MES 3.9 g       MgSO4·7H2O 0.049 g       CaCl2·2H2O 0.044 g       (NH4)2HPO4 0.0264 g       Trace element solution (SL-10)*** 2 ml       Se/W solution**** 2 ml       Distilled water 1 L   pH 5.5       Adjust pH to 5.5 with NaOH.                   ***Trace element solution (SL-10)       HCl (25%) 10 ml       FeCl2·4H2O 1.5 g       ZnCl2 0.07 g       MnCl2·4H2O 0.1 g       B(OH)3 0.006 g       CoCl2·6H2O 0.19 g       CuCl2·2H2O 0.002 g       NiCl2·6H2O 0.024 g       H4MoNa2O6·2H2O 0.036 g       Distilled water 1 L                   ****Se/W solution       NaOH 0.4 g       Na2SeO3 0.002 g       Na2WO4 0.004 g       Distilled water 1 L
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