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Medium Detail Information

Medium No.
1383
Medium
Halo 2a
Composition
 Bacto Yeast Extract (Difco)2g
 Casamino acids2g
 Sodium glutamate1g
 Trisodium citrate1g
 KCl2g
 K2HPO40.3g
 CaCl2·2H2O0.15g
 NH4Cl1g
 MgSO4·7H2O50g
 NaCl200g
 Trace elements solution*2ml
 Agar (if needed)20g
 Distilled water1L
 pH 7.0-7.4 
       
  *Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   FeCl3·6H2O1.35g
   MnCl2·4H2O0.1g
   CoCl2·6H2O0.024g
   CaCl2·2H2O0.1g
   ZnCl20.1g
   CuCl2·2H2O0.025g
   H3BO30.01g
   Na2MoO4·2H2O0.024g
   NaCl1g
   NiCl2·6H2O0.12g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
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