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Medium Detail Information

Medium No.	1408
Medium	1/10 PE medium
Composition
Basal salts solution* 5 ml   Phosphate solution** 5 ml   Vitamin solution*** 0.2 ml   (NH4)2SO4 0.05 g   Sodium glutamate 0.05 g   Sodium succinate 0.05 g   Sodium acetate 0.05 g   Yeast extract 0.05 g   Casamino acids 0.05 g   Na2S2O3·5H2O 0.05 g   Distilled water 1 L   pH 7.5       Add the vitamin and yeast extract solutions aseptically to the cooled autoclaved medium.                   *Basal salts solution       3Na-EDTA 4.53 g       FeSO4·7H2O 1.11 g       MgSO4·7H2O 24.65 g       CaCl2·2H2O 2.94 g       NaCl 23.4 g       MnSO4·4H2O 112 mg       ZnSO4·7H2O 29 mg       Co(NO3)2·6H2O 29 mg       CuSO4·5H2O 25 mg       Na2MoO4·2H2O 24 mg       H3BO3 31 mg       Distilled water 1 L                   **Phosphate solution       KH2PO4 75 g       K2HPO4 78 g       Distilled water 1 L                   ***Vitamin solution       3Na-EDTA 200 mg       Nicotinic acid 100 mg       Thiamine-HCl 100 mg       Biotin 5 mg       p-Aminobenzoic acid 50 mg       Vitamin B12 1 mg       Ca-pantothenate 50 mg       Pyridoxine-HCl 50 mg       Folic acid 50 mg       Distilled water 100 ml     Sterilized by filtration.
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