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Medium Detail Information

Medium No.
1412
Medium
Composition
 Trypticase peptone10g
 Yeast extract5g
 Glucose10g
 K2HPO40.45g
 KH2PO40.45g
 (NH4)2SO40.9g
 NaCl0.9g
 MgSO4·7H2O0.09g
 CaCl2·2H2O0.09g
 Resazurin1mg
 Na2CO30.2g
 Cysteine-HCl·H2O0.3g
 Distilled water1L
  Mix ingredients except cysteine-HCl. Dispense the medium into suitable culture vessels under a stream of N2 gas and seal with butyl rubber stoppers. Separately autoclave cysteine-HCl as 5% solution under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the cysteine-HCl solution. pH after autoclaving is 6.7-7.0.
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