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Medium Detail Information

Medium No.
1448 
Medium
Nitrosomonas mobilis medium 
Composition
 NH4Cl0.115g
 NaCl0.116g
 MgSO4·7H2O0.4g
 CaCl2·2H2O0.073g
 KCl0.038g
 KH2PO40.034g
 NaHCO3*0.84g
 Trace element solution**5ml
 Distilled water1L
       
  *Separately sterilize by filtration and aseptically add to the medium. Final pH is 8.
       
  **Trace elements solution
   Nitrilotriacetic acid (NTA)12.8g
   FeCl3·6H2O1.35g
   MnCl2·4H2O0.1g
   CoCl2·6H2O0.024g
   CaCl2·2H2O0.1g
   ZnCl20.1g
   CuCl2·2H2O0.025g
   H3BO30.01g
   Na2MoO4·2H2O0.024g
   NaCl1g
   NiCl2·6H2O0.12g
   Na2SeO40.004g
   Na2WO40.004g
   Distilled water1L
  First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
 
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