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Medium Detail Information

Medium No.	1448
Medium	Nitrosomonas mobilis medium
Composition
NH4Cl 0.115 g   NaCl 0.116 g   MgSO4·7H2O 0.4 g   CaCl2·2H2O 0.073 g   KCl 0.038 g   KH2PO4 0.034 g   NaHCO3* 0.84 g   Trace element solution** 5 ml   Distilled water 1 L                   *Separately sterilize by filtration and aseptically add to the medium. Final pH is 8.                   **Trace elements solution       Nitrilotriacetic acid (NTA) 12.8 g       FeCl3·6H2O 1.35 g       MnCl2·4H2O 0.1 g       CoCl2·6H2O 0.024 g       CaCl2·2H2O 0.1 g       ZnCl2 0.1 g       CuCl2·2H2O 0.025 g       H3BO3 0.01 g       Na2MoO4·2H2O 0.024 g       NaCl 1 g       NiCl2·6H2O 0.12 g       Na2SeO4 0.004 g       Na2WO4 0.004 g       Distilled water 1 L     First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
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