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Medium Detail Information

Medium No.	1452
Medium	RPL medium
Composition
Sodium pyruvate 1 g   Disodium succinate 1 g   Bacto Yeast Extract (Difco) 1 g   (NH4)2SO4 1 g   K2HPO4 0.6 g   KH2PO4 0.4 g   Na2S2O3·5H2O 0.5 g   NaCl 0.2 g   MgCl2·6H2O 0.2 g   CaCl2·2H2O 0.045 g   Trace element solution* 2 ml   Vitamin B12 0.02 mg   Agar (if needed) 18 g   Distilled water 1 L     Mix ingredients except for vitamin, adjust pH to 6.0, and autoclave under a N2 atmosphere at 121°C for 20 min. Aseptically add the filter-sterile vitamin solution after autoclaving.                   *Trace elements solution       Nitrilotriacetic acid (NTA) 12.8 g       FeCl3·6H2O 1.35 g       MnCl2·4H2O 0.1 g       CoCl2·6H2O 0.024 g       CaCl2·2H2O 0.1 g       ZnCl2 0.1 g       CuCl2·2H2O 0.025 g       H3BO3 0.01 g       Na2MoO4·2H2O 0.024 g       NaCl 1 g       NiCl2·6H2O 0.12 g       Distilled water 1 L     First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.
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