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Medium Detail Information

Medium No.
1475 
Medium
AF6 (pH4.0) 
Composition
 NaNO3140mg
 NH4NO322mg
 MgSO4·7H2O30mg
 KH2PO410mg
 K2HPO45mg
 CaCl2·2H2O10mg
 CaCO310mg
 Fe-citrate2mg
 Citric acid2mg
 Biotin2μg
 Thiamine-HCl10μg
 Vitamin B61μg
 Vitamin B121μg
 P IV Metals solution*5ml
 MES buffer400mg
 Agar (if needed)12g
 Distilled water1L
 pH 4.0 
       
  *P IV Metals solution
   FeCl3·6H2O19.6mg
   MnCl2·4H2O3.6mg
   ZnSO4·7H2O2.2mg
   CoCl2·6H2O0.4mg
   Na2MoO4·2H2O0.25mg
   Na2EDTA·2H2O100mg
   Distilled water1L
 
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