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Medium Detail Information

Medium No.
1558
Medium
Modified PYG Medium
Composition
  CaCl2·2H2O 0.09 g
  MgSO4·7H2O 0.09 g
  K2HPO4 0.45 g
  KH2PO4 0.45 g
  (NH4)2SO4 0.9 g
  NaCl 0.9 g
  Bacto Tryptone (Difco) 10 g
  Bacto Yeast Extract (Difco) 5 g
  Glucose 10 g
  Na2CO3 0.2 g
  Resazurin 1 mg
  Cysteine-HCl 0.3 g
  Agar (if needed) 15 g
  Distilled water 1 L
  Adjust pH to 7.3  
 
    Mix ingredients except glucose and Na2CO3, and autoclave under an N2 atmosphere. After autoclaving, add filter-sterile glucose and Na2CO3 solutions.
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