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Medium Detail Information

Medium No.	1606
Medium	PY4S medium
Composition
CaCl2·2H2O 0.09 g   MgSO4·7H2O 0.09 g   K2HPO4 0.45 g   KH2PO4 0.45 g   (NH4)2SO4 0.9 g   NaCl 0.9 g   Bacto Tryptone (Difco) 10 g   Bacto Yeast Extract (Difco) 5 g   Glucose 0.25 g   Cellobiose 0.25 g   Maltose 0.25 g   Soluble starch 0.25 g   Na2CO3 0.2 g   Resazurin 1 mg   Cysteine-HCl 0.3 g   Agar (if needed) 15 g   Distilled water 1 L   Adjust pH to 7.3         Mix ingredients except Na2CO3 and cysteine-HCl, and autoclave under an N2 atmosphere. After autoclaving, add filter-sterile Na2CO3 and separately auctoclaved cysteine-HCl solutions.
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