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Medium Detail Information

Medium No.	1612
Medium	SSE/HD1:10 MEDIUM
Composition
Peptone 0.5 g Yeast extract 0.25 g Glucose 0.1 g 2-(N-Morpholino)ethanesulfonic acid 1.95 g *SSE (double concentrated) 500 ml Distilled water 500 ml Agar (if needed) 15 g **Trace element solution SL-10 1 ml ***Vitamin solution 1 ml Comment *SSE (double concentrated) CaCl2 x 2H2O 0.29 g NH4Cl 0.11 g MgCl2 x 6 H2O 0.2 g (NH4)2SO4 0.2 g MgSO4 x 7H2O 0.74 g CaSO4 x 2H2O 0.86 g Ca(NO3)2 x 4H2O 0.24 g NaNO3 0.42 g KH2PO4 (100 mM solution) 0.5 ml FeSO4 x 7H2O 0.01 g K2SO4 0.09 g Distilled water 1000 ml **Trace element solution (SL-10) HCl (25%) 10 ml FeCl2·4H2O 1.5 g ZnCl2 0.07 g MnCl2·4H2O 0.1 g B(OH)3 0.006 g CoCl2·6H2O 0.19 g CuCl2·2H2O 0.002 g NiCl2·6H2O 0.024 g H4MoNa2O6·2H2O 0.036 g Distilled water 1000 ml ***Vitamin solution Biotin 2 mg Folic acid 2 mg Pyridoxine-HCl (Vitamin B6) 10 mg Thiamine-HCl·H2O (Vitamin B1) 5 mg Riboflavin (Vitamin B2) 5 mg Nicotinic acid 5 mg D-Ca-Pantothenate 5 mg Cyanocobalamin (Vitamin B12) 0.1 mg p-Aminobenzoic acid 5 mg Lipoic acid 5 mg Distilled water 1000 ml Adjust pH to 7.0 with NaOH.
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