カタログ検索結果 詳細情報
Medium Detail Information
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Medium No.
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1662 | ||||||||||||||||||||||||||||||
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Medium
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HM Buffer + prey strain (E. coli) | ||||||||||||||||||||||||||||||
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Composition
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For solid medium (double-layer agar plate)**
For liquid medium
*Prepare filter sterilized 0.5 M CaCl2 and 0.6 M MgCl2 solution first. Fill up 5ml of 0.5 M HEPES to 100 ml with UPW and adjust pH to 7.4, then sterilized by autoclaving. After autoclaving, add 600 μl of 0.5 M CaCl2 and 333 µl of 0.6 M MgCl2. **First, pour 15ml of Base agar into a petri dish and let it solidify. Place prey strain and predatory strain on top of Base agar, pour in Top agar, mix well and let solidify. ***Cultivate prey strain (E. coli) at overnight in 100 ml of R2A medium at 28°C with shaking at 120 rpm, wash with HM Buffer, and adjust the OD660 to 50 suspend in HM Buffer. |
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Comment
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