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Medium Detail Information

Medium No.	1711
Medium
Composition
Solution A* 500  ml   Solution B** 500  ml   25% (w/v) MgSO4·7H2O 1  ml   7% (w/v) CaCl2·2H2O 1  ml   2% (w/v) FeCl3·6H2O 1  ml         pH 4.8-5.0                 Sterilized Solutions A and B separately by autoclaving, then mixed them. Aseptically add 1ml of each of filter-sterilized 25% (w/v) MgSO4·7H2O solution, 7% (w/v) CaCl2·2H2O solution and 2% (w/v) FeCl3·6H2O solution, respectively, to the medium, and adjust the pH to 4.8-5.0.                 *Solution A             Carboxymethyl cellulose, sodium salt 2.5  g       (NH4)2SO4 1.3  g       KH2PO4 0.37  g       Distilled water 500  ml         pH 3.5                   **Solution B             Glucose 1.0  g       Yeast extract 0.25  g       Tryptone 0.9  g       Gellan gum (if needed) 12  g       Distilled water 500  ml
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