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Medium Detail Information

Medium No.
 Bacto PPLO Broth (Difco)21g
 Active horse serum200ml
 Fresh baker's yeast extract (25% solution)*100ml
 Distilled water700ml
  Horse serum and yeast extract are sterilized by filtration and added to PPLO broth autoclaved and cooled.
  *Boil 250 g of dried baker's yeast suspended in 1 L of distilled water for 30 min, remove yeast cells by centrifugation, and sterilize by filtration.

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