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Medium Detail Information

Medium No.
 281
Medium
Composition
 Yeast extract1g
 (NH4)2SO41.3g
 CaCl2·2H2O0.08g
 MgSO4·7H2O0.25g
 KH2PO40.28g
 Na2SiO3·9H2O0.3g
 Na2MoO4·2H2O0.03g
 FeSO4·7H2O0.02g
 Distilled water1L
 Adjust pH to 3.0 with 10N H2SO4 
  For solid medium, equal volumes of separately autoclaved double-strength liquid medium (pH 2.0) and 1.6% Gelrite (Kelco, Division of Merck & Co, Inc.) are mixed, after cooling to 45℃.
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