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Medium Detail Information

Medium No.
286
Medium
Composition
  (NH4)2SO4 3 g
  CaCl2·2H2O 0.08 g
  MgSO4·7H2O 0.25 g
  KH2PO4 0.28 g
  KCl 0.1 g
  Ca(NO3)2·4H2O 0.01 g
  Yeast extract* 0.2 g
  Sulfur (powder)** 10 g
  Distilled water 1 L
    Adjust pH to 1.5 to 2.5 with 6 N H2SO4.
             
    *Yeast extract is autoclaved separately as 10% solution.
             
    **Sulfur is sterilized separately by autoclaving for 60 min at 105℃ on each of 3 successive days and added after inoculation.
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