| | KH2PO4 | 0.28 | g |
| | NaNO3 | 0.12 | g |
| | Sodium thioglycolate | 0.05 | g |
| | Sodium tartrate | 0.37 | g |
| | Sodium succinate | 0.37 | g |
| | Sodium acetate | 0.05 | g |
| | Vitamin mixture* | 5 | ml |
| | Ferric quinate solution** | 2 | ml |
| | HMSS solution*** | 1 | ml |
| | Distilled water | 1 | L |
| | Adjust pH to 6.75 with 1 N NaOH | |
| | | | | | | |
| | | *Vitamin mixture |
| | | | Biotin | 0.2 | mg |
| | | | Ca-pantothenate | 40 | mg |
| | | | Folic acid | 0.2 | mg |
| | | | myo-Inositol | 200 | mg |
| | | | Niacin | 40 | mg |
| | | | p-Aminobenzoic acid | 20 | mg |
| | | | Pyridoxine-HCl | 40 | mg |
| | | | Riboflavin | 40 | mg |
| | | | Thiamin-HCl | 20 | mg |
| | | | Choline chloride | 100 | mg |
| | | | Cyanocobalamin | 2 | μg |
| | | | Distilled water | 1 | L |
| | | | | | | |
| | | **Ferric quinate solution |
| | | | FeCl3·6H2O | 0.45 | g |
| | | | Quinic acid | 0.19 | g |
| | | | Distilled water | 1 | L |
| | | | | | | |
| | | ***HMSS solution |
| | | | Nitrilotriacetic acid | 10 | g |
| | | | MgSO4·7H2O | 29.7 | g |
| | | | CaCl2·2H2O | 3.3 | g |
| | | | Na2MoO4·2H2O | 12.7 | mg |
| | | | FeSO4·7H2O | 99 | mg |
| | | | Metal 44 solution**** | 50 | ml |
| | | | Distilled water | 1 | L |
|
| | | Neutralize the nitrilotriacetic acid with KOH. Dissolve the remaining ingredients and adjust the pH to 7.2 with KOH. |
| | | | | | | |
| | | ****Metal 44 solution |
| | | | EDTA | 2.5 | g |
| | | | ZnSO4·7H2O | 10.95 | g |
| | | | FeSO4·7H2O | 5 | g |
| | | | MnSO4·nH2O | 1.54 | g |
| | | | CuSO4·5H2O | 0.392 | g |
| | | | CoCl2·6H2O | 0.203 | g |
| | | | Na2B4O7·10H2O | 0.177 | g |
| | | | Distilled water | 1 | L |
|
| | | Good growth can be obtained in semi-solid medium (1 g of agar in 1 liter). |