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Medium Detail Information

Medium No.
289
Medium
Composition
 KH2PO40.28g
 NaNO30.12g
 Sodium thioglycolate0.05g
 Sodium tartrate0.37g
 Sodium succinate0.37g
 Sodium acetate0.05g
 Vitamin mixture*5ml
 Ferric quinate solution**2ml
 HMSS solution***1ml
 Distilled water1L
 Adjust pH to 6.75 with 1 N NaOH 
       
  *Vitamin mixture
   Biotin0.2mg
   Ca-pantothenate40mg
   Folic acid0.2mg
   myo-Inositol200mg
   Niacin40mg
   p-Aminobenzoic acid20mg
   Pyridoxine-HCl40mg
   Riboflavin40mg
   Thiamin-HCl20mg
   Choline chloride100mg
   Cyanocobalamin2μg
   Distilled water1L
       
  **Ferric quinate solution
   FeCl3·6H2O0.45g
   Quinic acid0.19g
   Distilled water1L
       
  ***HMSS solution
   Nitrilotriacetic acid10g
   MgSO4·7H2O29.7g
   CaCl2·2H2O3.3g
   Na2MoO4·2H2O12.7mg
   FeSO4·7H2O99mg
   Metal 44 solution****50ml
   Distilled water1L
  Neutralize the nitrilotriacetic acid with KOH. Dissolve the remaining ingredients and adjust the pH to 7.2 with KOH.
       
  ****Metal 44 solution
   EDTA2.5g
   ZnSO4·7H2O10.95g
   FeSO4·7H2O5g
   MnSO4·nH2O1.54g
   CuSO4·5H2O0.392g
   CoCl2·6H2O0.203g
   Na2B4O7·10H2O0.177g
   Distilled water1L
  Good growth can be obtained in semi-solid medium (1 g of agar in 1 liter).
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