| KH2PO4 | 0.28 | g |
| NaNO3 | 0.12 | g |
| Sodium thioglycolate | 0.05 | g |
| Sodium tartrate | 0.37 | g |
| Sodium succinate | 0.37 | g |
| Sodium acetate | 0.05 | g |
| Vitamin mixture* | 5 | ml |
| Ferric quinate solution** | 2 | ml |
| HMSS solution*** | 1 | ml |
| Distilled water | 1 | L |
| Adjust pH to 6.75 with 1 N NaOH | |
| | | | | | |
| | *Vitamin mixture |
| | | Biotin | 0.2 | mg |
| | | Ca-pantothenate | 40 | mg |
| | | Folic acid | 0.2 | mg |
| | | myo-Inositol | 200 | mg |
| | | Niacin | 40 | mg |
| | | p-Aminobenzoic acid | 20 | mg |
| | | Pyridoxine-HCl | 40 | mg |
| | | Riboflavin | 40 | mg |
| | | Thiamin-HCl | 20 | mg |
| | | Choline chloride | 100 | mg |
| | | Cyanocobalamin | 2 | μg |
| | | Distilled water | 1 | L |
| | | | | | |
| | **Ferric quinate solution |
| | | FeCl3·6H2O | 0.45 | g |
| | | Quinic acid | 0.19 | g |
| | | Distilled water | 1 | L |
| | | | | | |
| | ***HMSS solution |
| | | Nitrilotriacetic acid | 10 | g |
| | | MgSO4·7H2O | 29.7 | g |
| | | CaCl2·2H2O | 3.3 | g |
| | | Na2MoO4·2H2O | 12.7 | mg |
| | | FeSO4·7H2O | 99 | mg |
| | | Metal 44 solution**** | 50 | ml |
| | | Distilled water | 1 | L |
|
| | Neutralize the nitrilotriacetic acid with KOH. Dissolve the remaining ingredients and adjust the pH to 7.2 with KOH. |
| | | | | | |
| | ****Metal 44 solution |
| | | EDTA | 2.5 | g |
| | | ZnSO4·7H2O | 10.95 | g |
| | | FeSO4·7H2O | 5 | g |
| | | MnSO4·nH2O | 1.54 | g |
| | | CuSO4·5H2O | 0.392 | g |
| | | CoCl2·6H2O | 0.203 | g |
| | | Na2B4O7·10H2O | 0.177 | g |
| | | Distilled water | 1 | L |
|
| | Good growth can be obtained in semi-solid medium (1 g of agar in 1 liter). |