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Medium Detail Information

Medium No.	335
Medium	Medium for Marine Flexibacteria
Composition
KNO3 0.5 g   Sodium glycerophosphate 0.1 g   Trace element solution* 1 ml   Tris (hydroxymethyl) aminomethane 1 g   Bacto Tryptone (Difco) 5 g   Yeast extract 5 g   Agar (if needed) 15 g   Seawater** 1 L   pH 7.0                     *Trace element solution       H3BO4 2.85 g       MnCl2·4H2O 1.8 g       FeSO4·7H2O 1.36 g       CuCl2·2H2O 26.9 mg       ZnCl2 20.8 mg       CoCl2·6H2O 40.4 mg       Na2MoO4·2H2O 25.2 mg       Sodium tartrate 1.77 g       Distilled water 1 L                   **Filtered, aged seawater or artificial seawater (Daigo's Artificial Seawater SP***).                   ***Wako Pure Chemical Ind., Ltd., Osaka, Japan.
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