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Medium Detail Information

Medium No.
 335
Medium
 Medium for Marine Flexibacteria
Composition
 KNO30.5g
 Sodium glycerophosphate0.1g
 Trace element solution*1ml
 Tris (hydroxymethyl) aminomethane1g
 Bacto Tryptone (Difco)5g
 Yeast extract5g
 Agar (if needed)15g
 Seawater**1L
 pH 7.0 
       
  *Trace element solution
   H3BO42.85g
   MnCl2·4H2O1.8g
   FeSO4·7H2O1.36g
   CuCl2·2H2O26.9mg
   ZnCl220.8mg
   CoCl2·6H2O40.4mg
   Na2MoO4·2H2O25.2mg
   Sodium tartrate1.77g
   Distilled water1L
       
  **Filtered, aged seawater or artificial seawater (Daigo's Artificial Seawater SP***).
       
  ***Wako Pure Chemical Ind., Ltd., Osaka, Japan.
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