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Medium Detail Information

Medium No.	372
Medium	MMYS-II Medium
Composition
Solution I* 500 ml   Solution II** 500 ml   pH 7.8       Separately autoclave Solutions I and II. Mix aseptically and adjust final pH to 7.7 - 7.8.                   *Solution I       (NH4)2SO4 1 g       NaCl 8.2 g       MgCl2·6H2O 0.2 g       CaCl2·2H2O 0.05 g       Trace element solution SL8*** 1 ml       Sodium DL-malate 3.6 g       Yeast extract 1 g       Na2S2O3·5H2O 0.5 g       Distilled water 500 ml                   **Solution II       20 mM Potassium phosphate buffer 500 ml   pH 7.8                     ***Trace element solution SL8       2Na-EDTA 5.2 g       FeCl2·4H2O 1.5 g       ZnCl2 70 mg       MnCl2·4H2O 100 mg       H3BO3 62 mg       CoCl2·6H2O 190 mg       Na2MoO4·2H2O 36 mg       CuCl2·2H2O 17 mg       NiCl2·6H2O 24 mg       Distilled water 1 L
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