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Medium Detail Information

Medium No.
372
Medium
MMYS-II Medium
Composition
 Solution I*500ml
 Solution II**500ml
 pH 7.8 
  Separately autoclave Solutions I and II. Mix aseptically and adjust final pH to 7.7 - 7.8.
       
  *Solution I
   (NH4)2SO41g
   NaCl8.2g
   MgCl2·6H2O0.2g
   CaCl2·2H2O0.05g
   Trace element solution SL8***1ml
   Sodium DL-malate3.6g
   Yeast extract1g
   Na2S2O3·5H2O0.5g
   Distilled water500ml
       
  **Solution II
   20 mM Potassium phosphate buffer500ml
 pH 7.8 
       
  ***Trace element solution SL8
   2Na-EDTA5.2g
   FeCl2·4H2O1.5g
   ZnCl270mg
   MnCl2·4H2O100mg
   H3BO362mg
   CoCl2·6H2O190mg
   Na2MoO4·2H2O36mg
   CuCl2·2H2O17mg
   NiCl2·6H2O24mg
   Distilled water1L
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