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Medium Detail Information

Medium No.
377
Medium
Oceanithermus medium
Composition
 KH2PO40.75g
 K2HPO40.78g
 MgCl2·6H2O0.36g
 CaCl2·2H2O0.1g
 NH4Cl0.54g
 NaHCO35g
 Trace element solution*10ml
 Vitamin solution**10ml
 Yeast extract2g
 NaNO31.7g
 NaCl20g
 Distilled water1L
 pH unadjusted 
  Mix ingredients, except NaHCO3 and vitamin solution. Dispense the medium into suitable culture vessels under a stream of N2 and seal with butyl rubber stoppers. After autoclaving, add filter-sterile NaHCO3 and vitamin solutions.
       
  *Trace elements solution
   2Na-EDTA0.64g
   MgSO4·7H2O6.2g
   MnSO4·4H2O0.55g
   NaCl1g
   FeSO4·7H2O0.1g
   CoCl2·6H2O0.17g
   CaCl2·2H2O0.13g
   ZnSO4·7H2O0.18g
   CuSO40.05g
   KAl(SO4)2·12H2O0.018g
   H3BO30.01g
   Na2MoO4·2H2O0.011g
   NiCl2·6H2O0.025g
  Dissolve the 2Na-EDTA in 500 ml of distilled water, and after adding the salts, make up to 1 L.
       
  **Vitamin solution
   Biotin2mg
   Folic acid2mg
   Pyridoxine-HCl10mg
   Thiamine-HCl5mg
   Riboflavin5mg
   Nicotinic acid5mg
   Ca-pantothenate5mg
   p-Aminobenzoic acid1mg
   Vitamin B120.01mg
   Distilled water1L
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