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Medium Detail Information

Medium No.
383
Medium
2.5% NaCl GYPFK Agar/Broth
Composition
  Solution A
   Glucose10g
   Hipolypepton*5g
   Yeast extract5g
   Extract Bonito*5g
   NaCl25g
   K2HPO410g
   Salt solution**5ml
   Agar (if needed)15g
   Distilled water900ml
 pH unadjusted 
  Solution B
   Na2CO33g
   Distilled water100ml
 pH unadjusted 
  Solutions A and B are separately sterilized by autoclaving and mix aseptically.
  Final pH around 8.5.
       
  *Wako Pure Chemical Ind., Ltd., Osaka, Japan.
       
  **Salt solution
   MgSO4·7H2O40g
   MnSO4·4H2O2g
   FeSO4·7H2O2g
   Distilled water1L
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